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"Sampel najis dan swab yang dikumpulkan pada peringkat akut penyakit ini adalah spesimen yang paling berguna untuk diagnosis makmal sebelum antibiotik diberikan. Sekiranya disyaki wabak kolera, agen penyebab yang paling biasa adalah V. cholerae O1. Sekiranya kumpulan sero V. cholerae O1 tidak diasingkan, makmal harus menguji V. cholerae O139. Walau bagaimanapun, jika kedua-dua organisma ini tidak diasingkan, sangat perlu menghantar spesimen najis ke makmal rujukan.[perlu rujukan]
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