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In page Amanda Fisher:

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Fisher showed in 1985 that molecular clones of HIV, contained within approximately 18kb of contiguous proviral DNA, were biologically active and generated cytopathic virus when introduced into primary human T-cells.[citation needed] This established that products of the viral genome itself, rather than a cofactor or contaminant, were capable of killing human T cells and were therefore potentially capable of mediating the immunosuppressive effect of HIV. It provided the basis for dissecting the molecular function of each of the viral genes and for developing DNA-based diagnostic tests for HIV infection.[citation needed]