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In page Registration, Evaluation, Authorisation and Restriction of Chemicals:

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An opinion in Nature in 2009 by Thomas Hartung and Constanza Rovida estimated that 54 million vertebrate animals would be used under REACH and that the costs would amount to €9.5 billion, set against the annual European industry annual turnover of €507 billion.[4] Hartung is the former head of European Centre for the Validation of Alternative Methods (ECVAM).[citation needed] In a news release, ECHA criticised assumptions made by Hartung and Rovida; ECHA's alternative assumptions reduced sixfold the number of animals.[5]