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In page Small intestinal bacterial overgrowth:

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The gold standard for detection of SIBO is aspiration and culture of fluid from the jejunum. More than 105 colony-forming units (more than 100,000 bacteria) per milliliter from the small bowel suggests SIBO.[1] The normal small bowel has less than 104 bacteria per millilitre.[citation needed] However, some experts consider aspiration of more than 103 positive if the flora is predominantly colonic-type bacteria, as these types of bacteria are considered pathological in excessive numbers in the small intestine. The reliability of aspiration in the diagnosis of SIBO has been questioned, as SIBO can be patchy, and the reproducibility can be as low as 38%. Some doctors factor in a patient's response to treatment as part of the diagnosis.[2]