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In page Nazi human experimentation:

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From about July 1942 to about September 1943, experiments to investigate the effectiveness of sulfonamide, a synthetic antimicrobial agent, were conducted at Ravensbrück.[1] Wounds inflicted on the subjects were infected with bacteria such as Streptococcus, Clostridium perfringens (a major causative agent in gas gangrene) and Clostridium tetani, the causative agent in tetanus.[2] Circulation of blood was interrupted by tying off blood vessels at both ends of the wound to create a condition similar to that of a battlefield wound. Researchers also aggravated the subjects' infection by forcing wood shavings and ground glass into their wounds. The infection was treated with sulfonamide and other drugs to determine their effectiveness.[citation needed]