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In page Eleanor of Austria:

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Manuel and Eleanor married on 16 July 1518. They had two children: the Infante Charles (born 18 February 1520 – died 15 April 1521) and the Infanta Maria (born 18 June 1521, who was later one of the richest princesses of Europe). Eleanor became a widow on 13 December 1521, when Manuel died of the plague. As queen dowager, Eleanor returned to her brother's court in Spain.[citation needed] Eleanor's sister Catherine later married Eleanor's stepson, John III of Portugal.