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In page House of Medici:

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In 1534, following a lengthy illness, Pope Clement VII died—and with him the stability of the Medici's "senior" branch. In 1535, Ippolito Cardinal de' Medici died under mysterious circumstances. In 1536, Alessandro de' Medici married Charles V's daughter, Margaret of Austria; however, the following year he was assassinated by a resentful cousin, Lorenzino de' Medici. The deaths of Alessandro and Ippolito enabled the Medici's "junior" branch to lead Florence.[citation needed]