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In page Polymerase chain reaction:
"One major limitation of PCR is that prior information about the target sequence is necessary in order to generate the primers that will allow its selective amplification.[1] This means that, typically, PCR users must know the precise sequence(s) upstream of the target region on each of the two single-stranded templates in order to ensure that the DNA polymerase properly binds to the primer-template hybrids and subsequently generates the entire target region during DNA synthesis.[citation needed]
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