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In page Philip II of Spain:

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Philip's fourth and final wife was his niece, Anna of Austria. Pope Pius V initially refused to grant Philip the dispensation needed to marry Anna, citing biblical prohibitions and the danger of birth defects. The pope reluctantly gave his permission when Philip threatened to abandon the Holy League in their fight against the Ottoman Turks.[1][2] By contemporary accounts, this was a convivial and satisfactory marriage (1570–1580) for both Philip and Anna. This marriage produced four sons and one daughter. Anna died of heart failure 8 months after giving birth to Maria in 1580.[citation needed]