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In page Theotokos:

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The Niceno-Constantinopolitan Creed of 381 affirmed the Christian faith on "one Lord Jesus Christ, the only-begotten Son of God, begotten of the Father before all worlds (æons)", that "came down from heaven, and was incarnate by the Holy Ghost and of the Virgin Mary, and was made man". Since that time, the expression "Mother of God" referred to the Dyophysite doctrine of the hypostatic union, about the uniqueness with the twofold nature of Jesus Christ God, which is both human and divine (nature distincted, but not separable nor mixed). Since that time, Jesus was affirmed as true Man and true God from all eternity.[citation needed]