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In page Cherokee:

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Linguistic analysis shows a relatively large difference between Cherokee and the northern Iroquoian languages, suggesting they had migrated long ago. Scholars posit a split between the groups in the distant past, perhaps 3,500–3,800 years ago.[1] Glottochronology studies suggest the split occurred between about 1500 and 1800 BCE.[2] The Cherokee say that the ancient settlement of Kituwa on the Tuckasegee River is their original settlement in the Southeast.[1] It was formerly adjacent to and is now part of Qualla Boundary (the base of the federally recognized Eastern Band of Cherokee Indians) in North Carolina.[citation needed]